|
KMID : 1177720120060010015
|
|
Journal of Alternatives to Animal Experiments
2012 Volume.6 No. 1 p.15 ~ p.20
|
|
|
Level of interleukin-6 production from keratinocyte cell line could be a potential candidate for screening skin sensitizing chemicals
|
|
Kim So-Nam
Yeo Kyeong-Uk
Kim Hyoung-Ah
Heo Yong
|
|
|
Abstract
|
|
|
|
HaCaT cell, a human keratinocyte cell line, was evaluated for its usefulness in sorting out chemicals with skin allergic potential. Interleukin-6 production was determined in HaCaT cell culture supernatants and cell lysates. HaCaT cells were cultured in the presence or absence of 4 doses, 0.01X, 0.1X, 0.5X, or 1X CV75 (75% cell viability concentration) of 3 sensitizing chemicals (dinitrochlorobenzene, hexyl cinnamic aldehyde, cobalt chloride) and 2 irritant chemicals (lactic acid, isopropanol) for 48 and 72 hours. IL-6 stimulation index (SI) was calculated through dividing IL-6 amount at each chemical concentration by IL-6 amount for vehicle control. When SI exceeding 3 is considered a threshold for categorizing into skin-sensitizer, dinitrochlorobenzene and hexyl cinnamic aldehyde were positive since SI was 7.36 and 3.03 for dinitrochlorobenzene and hexyl cinnamic aldehyde, respectively, for cell lysates obtained from 48 hour cultured-HaCaT cells. Two non-sensitizing chemicals, lactic acid and isopropanol, demonstrated SI value below 3, indicating negative to skin sensitizing potential. These results indicate that IL-6 production could be a biomarker for classification of chemicals as sensitizers or non-sensitizers.
|
|
|
KEYWORD
|
|
|
skin sensitization, keratinocyte cell line, HaCaT, interleukin-6, cell lysate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|